毛菊苣醇提物对高脂血症性脂肪肝大鼠作用机制初探

目的:目前,国内外研究集中在毛菊苣降糖降脂方面的作用,并已有相关药物上市,但实验表明毛菊苣在保肝降脂作用方面也具有很好的效果,本文旨在探讨毛菊苣醇提物治疗高脂血症性脂肪肝的作用机制。方法:采用高脂饮食喂饲大鼠复制高脂血症性脂肪肝大鼠模型,实验分组为模型组、毛菊苣醇提物治疗高剂量组和低剂量组、苯扎贝特对照组和正常对照组;以高、低剂量毛菊苣醇提物进行干预,然后测定各组大鼠血清及肝组织中乳酸脱氢酶(LDH)、低密度脂蛋白(LDL)、甘油三酯(TG)、胆固醇(TC)、丙二醛(MDA)等指标的含量。结果:模型组大鼠血清及肝组织中LDH、TG、TC、LDL、MDA等活性均较正常组明显升高(P0.05),说明高脂饲料造模成功;与模型组比较,测得各治疗组大鼠血清、肝组织中相应指标均明显降低(P0.05;或P0.1);毛菊苣醇提物高剂量组与阳性对照组相比无显著差异。结论:实验结果表明:通过高脂饮食饲喂SD大鼠建立高脂血症动物模型的实验是成功的;同时,毛菊苣醇提物能够使脂肪肝大鼠的体重降低,降低肝脏脂肪性病变,降低脂肪肝大鼠血清ALT、AST含量,降低大鼠血清TC、TG、LDL-c,升高HDL-c;对模型SD大鼠肝脏中异常升高的MDA、LDH、LD均有明显的降低作用;形态学检查显示,醇提物高剂组肝脏脂变程度明显减轻,脂滴数量减少,提示该药物具有保肝降脂的药理作用。

The MechanismUnderlying the Therapeutic Effect of Alcohol Extract of CichoriumGlundulosumBoiss et Hout on Hyperlipidemia in Rats

Objective:Cichorium glundulosum Boiss et Hout has been well demonstrated to have a role in decreasing blood glucose and lipid levels and some related drugs have been available on the market. In the hyperlipidemia-induced fatty liver disease, it has also been demonstrated to be of curative effect. The current study aims to discuss the underlying mechanism with alcohol extract of Cichorium glundulosum Boiss et Hout in hyperlipidemia rats.Methods:High fat diet feeding was used to create hyperlipidemia-induced fatty liver disease model in rats. The experimental rats were divided into model group, treatment groups (the alcohol extract of Sample with high and low group), control group with Benzalkonium Bate and normal control group. And then the levels of LDH, LDL, TG, TC, MDA and LD in serum and liver tissue were measured.Results:The LDH, TG, TC, LDL, MDA and LD lelves in model group were higher than those in normal group obviously ( P<0.05 ). Compared with the model group, the corresponding indicators were significantly reduced in the treated group (P<0.05; or P<0.1). The alcohol extract of Sample with high group was no significantly different compared with that of positive control group(the BenzalkoniumBate group).Conclusion:High-fat diet feeding can be used for the establishment of experimental animal model of hyperlipidemia in SD rats; the alcohol extract of Cichoriumglundulosum Boiss et Hout can lead to weight loss, reduce hepatic steatosis, decrease serum ALT, AST , TC, TG and LDL-c, increase HDL-c and suppress the abnormally elevated MDA, LDH, LD in SD rat liver, suggesting it has the pharmacological effects.