介绍一种兔VX2肿瘤的传代和接种方法及其应用经验和体会

目的:介绍一种兔VX2肿瘤的传代和接种方法及其应用经验和体会,从而更好的利用此模型进行生物医学研究。方法:从荷瘤新西兰大白兔取活性良好的肿瘤组织块,制备肿瘤细胞悬液,过滤后接种于健康成年新西兰兔左后肢肌肉内。通过一般观察、MRI和大体及病理学切片对肿瘤进行验证。结果:肿瘤传代和接种后生长良好,MRI、大体及组织学验证保持了VX2的肿瘤特点。结论:本研究介绍的兔VX2肿瘤的传代与接种方法稳定、可靠,值得大家推广和应用。

A Method for Passage and Inoculation of VX2 Tumor in Rabbits and ItsApplication Experiences

Objective： To introduce a method for passage and inoculation of VX2 tumor in rabbits and summarize the experiences with the purpose of its better usage in biomedical research. Method： The tumor cell suspensions were prepared from the tumor tissue with excellent viability which dissected fi＇om a New Zealand white rabbit bearing the transplantable VX2 carcinoma intramuscularly in the left hind limbs. The general observation, MRI examination, gross and pathological examination were performed to confirm the tumor features. Result： The growth of VX2 tumors was excellent after intramuscular passage and inoculation of VX2 tumor cell suspentions in the left hind limbs. Also, MRI finding, gross and pathological observation all confirmed the characteristics of VX2 tumor in this rabbits. Conclusion： The method for passage and inoculation of VX2 tumor in rabbits that introduced in this paper proves to be stable and reliable, and it is worthy of wide application in the related field.