大鼠铜梳烧伤模型建立方法的改良

目的:改良大鼠铜梳烧伤模型的建立方法,对比不同方法建立的烧伤模型的烧伤间隙区初始面积及坏死程度的差异,探索更加理想的大鼠铜梳烧伤模型的建立方法。方法:48只SD大鼠被随机分为A组(原方法)、B组(改良法1)及C组(改良法2)。将铜梳烧伤器加热后,即刻置于各组大鼠背部中线两侧的皮肤上,A组铜梳接触皮肤时,除自身重力以外,不施加任何压力;B组铜梳接触皮肤时施加压力,使皮肤凹陷约0.5 cm;C组使用铜梳烧伤器嵌合聚乙烯泡沫塑料模型后再接触皮肤,并施加压力,使皮肤凹陷约0.5 cm。测量各组伤后即刻的烧伤间隙区面积;伤后6、12、24、48小时的间隙区组织坏死面积;各个时间点取材后,通过HE染色观察间隙区组织坏死程度并测量坏死深度。结果:伤后即刻C组的烧伤间隙区面积介于A组与B组之间,且与理论值最接近;B组间隙区组织坏死进程最快,伤后24小时已基本坏死。A组坏死进程最慢,间隙区组织基本坏死时间超过48小时。C组间隙区组织基本坏死时间是伤后48小时,与理论上的时间最接近。结论:通过铜梳烧伤器嵌合聚乙烯泡沫塑料模型后再接触皮肤,并施加压力,使皮肤凹陷约0.5 cm,是建立大鼠铜梳烧伤模型更加理想的方法。

Reformation of Building Method of Brass CombBurn Model in Rats*

ABSTRACT Objective:To reformthe method of building brass comb burn model in rats, and explore a more applicable method bycomparing the initial areas as well as necrosis degrees of the interspace in the models. Methods:48 rats were randomly divided intoGroupA (original method), GroupB (reformed method 1) and GroupC (reformed method 2). The brass comb burn model was built onboth sides of the rats'' back in each group with the heated comb brass. In Group A, there was no contact pressure provided to the skinsurface except the weight of the comb brass. In Group B, the pressure was provided to concave the skin for nearly 0.5 cm. In Group C,the heated comb brass combined with the polyethylene plastic model before it contacted the skin surface. Then the pressure was providedto concave the skin for nearly 0.5 cm. Then the initial interspace of each group was measured. Specimens were taken after measuring thenecrosis area of interspace at 6, 12, 24, 48h post burn. HE staining was applied to show the necrosis degree of the interspace and thenecrosis depth was measured.Results: The initial area of the interspace in Group C was the closest to the theory value, compared withGroup A and Group B, and the standard deviation was smaller than the others. The necrosis process in Group B was the fastest and theinterspace basically necrosed 24 h post burn. The necrosis process in Group A, developing beyond 48h post burn, was the slowest.Thegeneral necrosis in Group C was basically developed at 48 h post burn, which was closest to the time in theory. Conclusion:The heatedcomb brass combined with the polyethylene plastic model before contacting the skin surface and the pressure was then provided toconcave the skin for nearly 0.5cm, which was a more ideal method to build brass comb burn model in rats.