Rapid Identification of Viable Bacterial Spores Using a Fluorescence Method |
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Authors: | Dinesh K Sharma D N Prasad |
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Institution: |
a Dairy Microbiology Division, National Dairy Research Institute, Karnal, India
b Research & Development (PT), National Dairy Development Board, India |
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Abstract: | A method has been devised to differentiate viable and nonviable bacterial spores. “Germination-like” changes are initiated in spores with performic acid and lysozyme. The germinated spores are stained with aqueous acridine orange, a fluorescent dye. Nonviable spores fluoresce lemon-green and viable spores orange-red. It is proposed that with the use of a membrane filter resistant to performic acid and lysozyme, the method may be used for spore enumeration in foods in about 4 hr compared to conventional plating methods, which usually require up to 72 hr. |
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Keywords: | viable-nonviable spores germination-like changes differentiation UHT conventional plating |
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