Fenpropimorph slows down the sterol pathway and the development of the arbuscular mycorrhizal fungus Glomus intraradices |
| |
Authors: | E Campagnac J Fontaine A Lounès-Hadj Sahraoui F Laruelle R Durand A Grandmougin-Ferjani |
| |
Institution: | 1. Laboratoire de Mycologie Phytopathologie Environnement, Université du Littoral C?te d’Opale, 17 Avenue Blériot, B.P. 699, 62228, Calais Cedex, France
|
| |
Abstract: | The direct impact of fenpropimorph on the sterol biosynthesis pathway of Glomus intraradices when extraradical mycelia alone are in contact with the fungicide was investigated using monoxenic cultures. Bi-compartmental
Petri plates allowed culture of mycorrhizal chicory roots in a compartment without fenpropimorph and exposure of extraradical
hyphae to the presence of increasing concentrations of fenpropimorph (0, 0.02, 0.2, 2, 20 mg l−1). In the fungal compartment, sporulation, hyphal growth, and fungal biomass were already reduced at the lowest fungicide
concentration. A decrease in total sterols, in addition to an increase in the amount of squalene and no accumulation of abnormal
sterols, suggests that the sterol pathway is severely slowed down or that squalene epoxidase was inhibited by fenpropimorph
in G. intraradices. In the root compartment, neither extraradical and intraradical development of the arbuscular mycorrhizal (AM) fungus nor
root growth was affected when they were not in direct contact with the fungicide; only hyphal length was significantly affected
at 2 mg l−1 of fenpropimorph. Our results clearly demonstrate a direct impact of fenpropimorph on the AM fungus by a perturbation of
its sterol metabolism. |
| |
Keywords: | Glomeraceae Arbuscular mycorrhiza Lipid Sterol Sterol biosynthesis inhibitor (SBI) fungicides Monoxenic cultures |
本文献已被 SpringerLink 等数据库收录! |
|