Identification of mutans streptococci with monoclonal antibodies |
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Authors: | J J de Soet P J van Dalen R R B Russell J de Graaff |
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Institution: | (1) Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam (ACTA), van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands;(2) Hunterian Dental Research Unit, London Hospital Medical College, E1 2AD London, UK |
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Abstract: | Mutans streptococci have been correlated with dental caries. The identification of the species within this group is still a problem. The characterization of a monoclonal antibody (Mab) OMVU10 against S. sobrinus as well as the isolation and characterization of Mabs against S. mutans (OMVU30 and OMVU31), S. cricetus (OMVU40) and mutans streptococci (OMVU2) is described. The epitope specificity for OMVU10 and OMVU31 was cell-wall antigen B in both cases although both Mabs recognized different species-specific epitopes. OMVU40 was cross reactive with Steptococcus sanguis taxon 3. All other Mabs were specific for one species. Using these Mabs, a key to the identification of mutans streptococci is developed. This key was tested for 85 wild type isolates of mutans streptococci and proved to be highly reliable and easy to perform. |
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Keywords: | identification monoclonal antibodies mutans streptococci S sobrinus S mutans |
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