Cloning of E. coli penicillin G acylase gene with mini-Mu containing a plasmid replicon |
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| Authors: | Andrea Laczaová Tibor Pechan Stanislav Stuchlík Renáta Kormutáková Ján Turňa |
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| Affiliation: | (1) Department of Molecular Biology, Comenius University, 842 15 Bratislava, Slovakia |
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| Abstract: | Summary An in vivo cloning system based on mini-Mu derivatives was used for cloning of E. coli penicillin G acylase gene (pac). We have constructed several recombinant clones producing penicillin G acylase and some of them exhibit approximately two times higher activity than original strains. |
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