MicroRNA‐34/449 controls mitotic spindle orientation during mammalian cortex development |
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Authors: | Juan Pablo Fededa Christopher Esk Beata Mierzwa Rugile Stanyte Shuiqiao Yuan Huili Zheng Klaus Ebnet Wei Yan Juergen A Knoblich Daniel W Gerlich |
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Institution: | 1. Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), Vienna, Austria;2. Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, USA;3. Institute‐associated Research Group “Cell Adhesion and Cell Polarity”, Institute of Medical Biochemistry, ZMBE, Münster, Germany |
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Abstract: | Correct orientation of the mitotic spindle determines the plane of cellular cleavage and is crucial for organ development. In the developing cerebral cortex, spindle orientation defects result in severe neurodevelopmental disorders, but the precise mechanisms that control this important event are not fully understood. Here, we use a combination of high‐content screening and mouse genetics to identify the miR‐34/449 family as key regulators of mitotic spindle orientation in the developing cerebral cortex. By screening through all cortically expressed miRNAs in HeLa cells, we show that several members of the miR‐34/449 family control mitotic duration and spindle rotation. Analysis of miR‐34/449 knockout (KO) mouse embryos demonstrates significant spindle misorientation phenotypes in cortical progenitors, resulting in an excess of radial glia cells at the expense of intermediate progenitors and a significant delay in neurogenesis. We identify the junction adhesion molecule‐A (JAM‐A) as a key target for miR‐34/449 in the developing cortex that might be responsible for those defects. Our data indicate that miRNA‐dependent regulation of mitotic spindle orientation is crucial for cell fate specification during mammalian neurogenesis. |
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Keywords: | cortex development miR‐449 neurogenesis radial glia spindle orientation |
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