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Structural role of the T94I rhodopsin mutation in congenital stationary night blindness
Authors:Ankita Singhal  Ying Guo  Milos Matkovic  Gebhard Schertler  Xavier Deupi  Elsa CY Yan  Joerg Standfuss
Institution:1. Division of Biology and Chemistry, Laboratory of Biomolecular Research, Paul Scherrer Institute, Villigen, Switzerland;2. Department of Chemistry, Yale University, New Haven, CT, USA;3. Deparment of Biology, ETH Zurich, Zürich, Switzerland;4. Condensed Matter Theory Group, Paul Scherrer Institute, Villigen, Switzerland
Abstract:Congenital stationary night blindness (CSNB) is an inherited and non‐progressive retinal dysfunction. Here, we present the crystal structure of CSNB‐causing T94I2.61 rhodopsin in the active conformation at 2.3 Å resolution. The introduced hydrophobic side chain prolongs the lifetime of the G protein activating metarhodopsin‐II state by establishing a direct van der Waals contact with K2967.43, the site of retinal attachment. This is in stark contrast to the light‐activated state of the CSNB‐causing G90D2.57 mutation, where the charged mutation forms a salt bridge with K2967.43. To find the common denominator between these two functional modifications, we combined our structural data with a kinetic biochemical analysis and molecular dynamics simulations. Our results indicate that both the charged G90D2.57 and the hydrophobic T94I2.61 mutation alter the dark state by weakening the interaction between the Schiff base (SB) and its counterion E1133.28. We propose that this interference with the tight regulation of the dim light photoreceptor rhodopsin increases background noise in the visual system and causes the loss of night vision characteristic for CSNB patients.
Keywords:congenital stationary night blindness  constitutive activity  G protein‐coupled receptors  rhodopsin  visual system
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