Scanning electron microscopy of immobilizedHumicola lutea during semicontinuous production of acid proteinases |
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Authors: | L Michailova P Aleksieva |
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Institution: | (1) Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev St., B1.26, 1113 Sofia, Bulgaria |
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Abstract: | The morphology of the fungusHumicola lutea (strain 120–5), immobilized in polyacrylamide and polyhydroxyethylmethacrylate and used for the semicontinuous production
of acid proteinases, was examined by scanning electron microscopy. The fungus developed a dense mycelium below the bead surface
as well as in the bead interior after precultivation of entrapped spores. During maximal semicontinuous enzyme biosynthesis,
formation of numerous large bulbous cells with a different shape was observed. Lysis of the cells was observed mainly in the
centre of the gel beads after 13 successive fermentations with polyacrylamide-immobilized cells or after 21 re-uses of polyhydroxyethylmethacrylate-immobilized
mycelia, respectively. Growth and changes in the cellular morphology of immobilizedH. lutea, accompanying biosynthesis of acid proteinases, were comparable in both gel matrices but mycelia immobilized in polyhydroxyethylmethacrylate
maintained their productivity twice as long. |
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Keywords: | Acid proteinase Humicola lutea immobilization scanning electron microscopy |
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