Lactic acid from acid whey permeate in a membrane recycle bioreactor |
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Affiliation: | 1. Laboratorio de Futuros en Bioenergía, Centro de Investigación y de Estudios Avanzados del IPN Unidad Guadalajara, Av. Del Bosque # 114, CP 45019, Jalisco, México;1. Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;2. Maize research institute “Zemun polje”, Slobodana Bajića, 11185 Belgrade, Serbia;3. Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia |
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Abstract: | Whey permeate was obtained by ultrafiltration of cottage cheese whey and supplemented with yeast extract. The lactose in the permeate was converted into lactic acid by Lactobacillus bulgaricus in a high-performance membrane bioreactor configured in the cell recycle mode. At a cell concentration of 10 g l−1, optimum productivity of lactic acid was 35 g l−1 h−1. Increasing the cell concentration to 30 g l−1 enabled the use of a dilution rate of 1 h−1 with complete substrate utilization. At 60 g l−1, productivity was over 80 g l−1 h−1 with complete substrte utilization; this is vastly superior to conventional batch fermentations. |
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