Production of cellulases and d-xylanase by some selected fungal isolates |
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| Institution: | 1. College of Food Science and Technology, Hunan Agricultural University, East Renmin Road, Changsha 410128, Hunan, China;2. Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan, China;3. College of Horticulture and Gardening, Hunan Agricultural University, East Renmin Road, Changsha 410128, Hunan, China;1. Department of Medicinal Chemistry, Chia Nan University of Pharmacy and Science, 60, Sec. 1, Erren Rd., Rende Dist., Tainan City 71710, Taiwan;2. Department of Environmental Engineering, Chiang Mai University, Chiang Mai 50200, Thailand;3. Department of Environmental Engineering and Science, Chia Nan University of Pharmacy and Science, 60, Sec. 1, Erren Rd., Rende Dist., Tainan City 71710, Taiwan;4. Indoor Air Quality Research and Service Center, Chia Nan University of Pharmacy and Science, 60, Sec. 1, Erren Rd., Rende Dist., Tainan City 71710, Taiwan;1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;3. Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu 808-0196, Japan |
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| Abstract: | Cellulase see 1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4], β-d-glucosidase (β-d-glucoside glucanohydrolase, EC 3.2.1.21) and d-xylanase (1,4-β-d-xylan xylanohydrolase, EC 3.2.1.8) production by Aspergillus ustus, Sporotrichum pulverulentum, Trichoderma sp. (a), Trichoderma sp. (b) and Botrytis sp. in solid state fermentation on different compounded media containing wheat bran (WB), rice straw (RS) and minerals was studied. Toyama's mineral solution mixed with RS was found to be a better substrate for cellulase and d-xylanase while with WB it induced higher β-d-glucosidase production. A ratio of substrate to mineral solution (w/v) of 1:4 or 1:5 supported high d-xylanase and cellulase production whereas a ratio of 1:2 gave the highest β-d-glucosidase activity. Among the fungal isolates, Aspergillus ustus gave the highest β-d-glucosidase activity of 60 U g−1WB and the highest d-xylanase activity of 740 U g−1was obtained with RS. A mixture of seven parts of RS and three of WB, mixed with 40 parts of Toyama's mineral solution yielded 6 U filter paper activity, 40 U β-d-glucosidase, 12 U carboxymethylcellulase and 650 U d-xylanase g−1substrate. |
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