| Abstract: | DNA-dependent RNA polymerase activity has been measured in unfertilized sea urchin eggs and swimming blastula stage embryos. The total activity detectable at these two stages was found to be comparable. Assays of isolated nuclei and non-nucleate half preparations indicate that the activity in the unfertilized egg is localized in the cytoplasm, in contrast to what is observed in the blastula stage empryo cell where the activity is found exclusively in the nucleus. Similar DEAE-sephadex chromatography profiles are obtained with enzyme isolated from either source. |
