Catechin production in cultured cells of Taxus cuspidata and Taxus baccata |
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Authors: | Bulgakov Victor P Tchernoded G K Veselova M V Fedoreyev S A Muzarok T I Zhuravlev Y N |
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Affiliation: | (1) Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 159 Stoletija Str, Vladivostok, 690022, Russia;(2) Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, 690022, Russia |
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Abstract: | The main polyphenols in callus and cell suspension cultures of Taxus cuspidata and T. baccata were (+)-catechin and (−)-epicatechin, while lignans, such as (+)-taxiresinol, (+)-isotaxiresinol, (+)-isolariciresinol and (−)-secoisolariciresinol, were present in trace amounts. T. cuspidata cells contained 1.7% (+)-catechin and 2.4% (−)-epicatechin on dry wt basis but when stimulated with methyl jasmonate produced 3.4% catechin and 5.2% epicatechin. These are the highest levels of these metabolites obtained in plant cell cultures. |
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