Effect of ammonium ions on ?5,7-sterol synthesis inSaccharomyces cerevisiae |
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| Authors: | C Novotný K Beran B Běhalová L Dolezalová J Zají?ek |
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| Institution: | (1) Department of Enzyme Engineering, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4;(2) Department of Microbial Technology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
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| Abstract: | The effect of ammonium concentration in the medium on †5,7-sterol synthesis was examined. Higher concentrations of this nitrogen source in the medium decreased sterol synthesis and
accumulation during growth. An intermittent supply with ammonium resulted in a proportional synthesis of †5,7-sterols and biomass. The carbon to nitrogen molar ratio of ≧ 40 allowed the maximum accumulation of †5,7-sterols with our strain of baker’s yeast. |
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