Automatic cellulase assay in computer coupled pilot fermentation

Equipment for the automatic measurement of cellulase activity was connected to a computer-coupled pilot fermenter. The measurement was based on the use of dyed Avicel cellulose as substrate. An intermittent sampling device was used to take a sample from the fermenter at intervals of one hour. The computer was programmed to calculate the cellulase activity during fermentation. The measured activity values were corrected against a standard sample of known activity by means of a mathematical model for the calibration curve which was stored in the computer. On-line measurement of enzyme activity was successfully performed.