|
|||||
|
|
| 进口肉骨粉中牛成分检测研究 | |
| 引用本文： | 潘良文,陈家华,丁燕,潘爱虎,袁政,梁婉琪,张大兵. 进口肉骨粉中牛成分检测研究[J]. 生物技术通报, 2001, 0(5): 23-26 |
| 作者姓名： | 潘良文 陈家华 丁燕 潘爱虎 袁政 梁婉琪 张大兵 |
| 作者单位： | 1. 上海出入境检验检疫局 2. 上海水产大学食品学院2001届毕业实习生 3. 上海农业科学研究院生物技术研究中心 |
| 基金项目： | 本项工作得到上海市科委重点项目资助,项目号为:003912075. |
| 摘 要： | 根据18S核糖体基因保守序列设计引物，对牛骨粉，鱼粉，牛肉，羊肉，猪肉，鸡肉，鸭肉，鱼肉的DNA样品进行PCR扩增，均得到137bp的扩增片段；根据牛线粒体的特异基因片段设计引物，对上述材料DNA样品进行PCR扩增，结果只在牛肉和牛骨粉中出现271bp的扩增，本实验建立了从进口肉骨粉中检测牛成分的PCR方法，检测灵敏度达到牛成分含量为0．1％（W／W）的水平，用建立起来的方法对进口肉骨粉，鱼粉和饲料添加剂进行了检测，结果在5批肉骨粉中检测出含有可能导致疯牛病的牛成分。 |
| 关 键 词： | 疯牛病 肉骨粉 牛成分 线粒体DNA PCR 检测 |
Research on Detection of Bovine Material From Imported Meat and Bone Meal |
|
| Pan Liangwen Chen jiahua Ding Yan Pan Aihu Yuan Zheng Liang Wanqi Zhang Dabing. Research on Detection of Bovine Material From Imported Meat and Bone Meal[J]. Biotechnology Bulletin, 2001, 0(5): 23-26 | |
| Authors: | Pan Liangwen Chen jiahua Ding Yan Pan Aihu Yuan Zheng Liang Wanqi Zhang Dabing |
| Affiliation: | Pan Liangwen 1 Chen jiahua 1 Ding Yan 2 Pan Aihu 3 Yuan Zheng 3 Liang Wanqi 3 Zhang Dabing 3 1 |
| Abstract: | The keephousing sequence of 18S ribosomal gene of the meat and bone meal(MBM), fish meal, the fresh meat of bovine, sheep, porcine, chicken, duck and fish were detected by PCR with a amplicon of 137 bp for each sample.The mitochondrial gene of the above samples were also detected by PCR with a pair of bovine-specific primers, only amplicons of 271 bp appeared from the bovine and MBM. A method of detection bovine material from MBM is conducted with the sensetivity of 0.1%.The method was applied to the detection of imported MBM, fish meal and feedstuffs additives,and the bovine material were found in 5 shipment of MBM. |
| Keywords: | Bovine Spongiform Encephalopathy(BSE) Meat and Bone Meal(MBM) Bovine Mitochondrial DNA Polymerase Chain Reaction (PCR) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |