Induction of T-cell proliferation and enhancement of NK activity by supernatants from Con A-stimulated human peripheral blood mononuclear cells: a new lymphokine |
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| Authors: | E F Rosloniec R P Cleveland D B Kaufman |
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| Institution: | 1. CAS Key Laboratory of Innate Immunity and Chronic Diseases, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei 230027, China;2. Center for Personal Dynamic Regulomes and Program in Epithelial Biology, Stanford University School of Medicine, CCSR 2155c, 269 Campus Drive, Stanford, CA 94305-5168, USA;3. Department of Dermatology, Stanford University School of Medicine, Stanford, CA 94305, USA;4. Epinomics, Menlo Park, CA 94035, USA;5. Stanford Blood and Marrow Transplantation Cellular Therapy Facility, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA;6. CNRSUMR 5164, Université de Bordeaux, Bordeaux 33076, France;7. Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA;8. Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA;1. Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3204, USA;2. HHMI Mass Spectrometry Laboratory, University of California, Berkeley, Berkeley, California 94720-3204, USA;1. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University) of Ministry of Education, Chengdu, Sichuan, China;2. Department of Obstetrics and Gynecology, Nanchong Central Hospital, Nanchong, Sichuan, China;1. Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA |
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| Abstract: | Supernatants from human peripheral blood mononuclear cells activated by Con A contain a factor(s) that stimulates blastogenic activity of normal human peripheral blood mononuclear cells. This Con A supernatant (CAS) contains stimulatory activity for E-rosette positive lymphocytes (T cells) and requires adherent cells for stimulation of T-cell proliferation. CAS does not contain detectable amounts of IL-2 as determined by its inability to support CTLL cell growth. Nor does it contain IL-1 or interferon. Examination of functional activity of lymphocytes stimulated for 3 days by CAS revealed that NK activity is augmented. This supernate does not appear to have any direct effect on B-cell function, although it induces suppression of polyclonal PWM stimulation of immunoglobulins. Thus, CAS appears to contain a new cytokine with immunomodulating potential. |
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