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棋盘稀释法在解脲脲原体药敏试验中的应用
引用本文:黄亚,郭晓奎,李擎天.棋盘稀释法在解脲脲原体药敏试验中的应用[J].中国微生态学杂志,2010,22(10):915-917.
作者姓名:黄亚  郭晓奎  李擎天
作者单位:[1]上海交通大学医学院病原生物学教研室,上海200025 [2]上海交通大学医学院附属瑞金医院检验系,上海200025
基金项目:"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项
摘    要:解脲脲原体是引起泌尿生殖道感染的重要病原体。目前使用的药敏试验方法即药敏卡法没有考虑样本中脲原体的实际含量而存在局限性。本研究将棋盘稀释法应用于解脲脲原体的药敏试验,可获得脲原体的准确耐药情况,有助于临床治疗中抗生素的正确选择;同时,有利于控制耐药菌株的产生和在人群中的扩散。

关 键 词:棋盘稀释法  解脲脲原体  药敏试验

The checkerboard dilution method in the susceptibility test for Ureaplasma urealyticum
HUANG Ya,GUO Xiao-kui,LI Qing-tian.The checkerboard dilution method in the susceptibility test for Ureaplasma urealyticum[J].Chinese Journal of Microecology,2010,22(10):915-917.
Authors:HUANG Ya  GUO Xiao-kui  LI Qing-tian
Institution:1.Department of Medical Microbiology and Parasitology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;2.Department of Laboratory Medicine,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Slanghai 200025,China)
Abstract:Ureaplasma urealyticum is a major pathogen which caused urogenital infections.There are limitations in the current Mycoplasma IST2 susceptibility test which doesn′t consider the actual content in the specimen.In this study,the checkerboard dilution method was applied to the susceptibility test for Ureaplasma urealyticum;the accurate resistance results could be used in the correct choice of antibiotics in the clinical treatment.At the same time,it can help control the production and the spread in the population of resistant strains.
Keywords:Checkerboard dilution method  Ureaplasma urealyticum  Susceptibility test  
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