Hot spots of DNA instability revealed through the study of somaclonal variation in rye |
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Authors: | R Linacero E Freitas Alves A M Vázquez |
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Institution: | (1) Departamento de Genética, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain, ES |
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Abstract: | RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature embryos and inflorescences.
From the studied plants, 40% showed at least one variation, and the number of mutations per plant was quite high, ranging
from 1 up to 12. On some occasions (2.9% of the scored bands) the modified band was observed in only one plant or in several
but originated from the same callus (variable band). In other cases (5.25%) the same band varied in several plants obtained
from different calli. We call these hypervariable bands and they could vary between plants belonging to different cultivars
and/or with different origins, inflorescences or embryos. Thus, they must originate through independent mutational events.
We assume that these bands represent hypervariable regions of the rye genome and so detect hot spots of DNA instability. Some
of these bands proved to be unique sequences, others were present in a low copy number while the remaining ones were moderately
or highly repetitive.
Received: 10 May 1999 / Accepted: 17 June 1999 Communicated by R. Hagemann |
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Keywords: | Rye DNA instability Hypervariable sequences Somaclonal variation RAPD |
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