Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis |
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| Authors: | A Roy N Frascaria J MacKay J Bousquet |
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| Institution: | (1) Centre de recherche en biologie Forestière, Faculté de foresterie et de géomatique, Université Laval, G1K 7P4 Sainte-Foy, Québec, Canada;(2) Ministère des Forêts du Québec, Direction de la recherche, 2700 Einstein, G1P 3W8 Ste-Foy, Québec, Canada;(3) Present address: Department of Forestry, North Carolina State University, P.O. Box 8008, NC 27695-8008 Raleigh, USA |
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| Abstract: | Summary Molecular markers are currently being developed for Betula alleghaniensis Britton using random amplified polymorphic DNA (RAPD). Arbitrarily designed 11-mer primers were tested on three intraspecific controlled crosses for which more than 15 full-sibs were available. Using two of these primers, we were able to genetically characterize a total of nine polymorphic RAPD markers. Segregation of these markers was consistent with a biparental diploid mode of inheritance, and all appeared dominant. RAPDs were valuable in detecting contaminants and, therefore, in assessing the validity of controlled crosses. Limitations of the technique are discussed in relation to the determination of parental genotypes and construction of linkage maps for hardwood species. |
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| Keywords: | RAPD Betula alleghaniensis Segregation analysis Genetic markers Controlled crosses |
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