|
|||||
|
|
Expression of a functional recombinant chimeric protein of human hepatitis A virus VP1 and an Fc antibody fragment in transgenic tomato plants |
|
| Authors: | Ho Yong Chung Jong-Hwa Park Hyun Ho Lee Kyung Il Kim Ha Young Chung Garry Sunter Jong Bum Kim Wonyong Kim In Sik Chung |
| Institution: | 1. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea 2. Department of Biology, University of Texas at San Antonio, San Antonio, TX, 78249, USA 3. Functional Biomaterial Division, National Academy of Agricultural Science, RDA, Suwon, 441-707, Korea 4. Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, Seoul, 156-756, Korea |
| Abstract: | Transgenic tomato plants expressing the gene of a chimeric protein (HAV VP1-Fc) consisting of human hepatitis A virus (HAV) VP1 and an Fc antibody fragment have been obtained. Recombinant VP1-Fc protein with a molecular mass of approximately 68 kDa was purified from transgenic tomato plants using Protein A Sepharose affinity chromatography. The recombinant protein elicited production of specific IgG antibodies in the serum after intraperitoneal immunization of BALB/c mice. The antibodies produced by mice against transgenic plant-derived recombinant VP1-Fc most likely recognize epitopes in the HAV viral antigen. Following vaccination with recombinant VP1-Fc protein, expression of IFN-γ and IL-4 were increased in splenocytes at the time of sacrifice. Our findings indicate that transgenic tomato plants can provide a useful system for the production of HAV antigens. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |