The establishment of new cell lines from Pseudaletia unipuncta with differential responses to baculovirus infection |
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Authors: | Ping Wang Roger Toung Robert R Granados |
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Institution: | (1) Department of Entomology, Cornell University, 14853 Ithaca, New York;(2) Boyce Thompson Institute, Cornell University, 14853 Ithaca, New York |
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Abstract: | Summary Six insect cell lines from Pseudaletia unipuncta embryos were established and characterized, and their susceptibility to Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infection was investigated. These embryonic P. unipuncta cell lines had characteristics distinct from each other in morphology and growth, and showed differential responses to AcMNPV
infection. Among the six cell lines, two were highly susceptible to virus infection. One of these two cell lines, BTI-Pu-A7S,
produced over 100 AcMNPV occlusion bodies per cell, on average. Three cell lines showed an apoptotic response following AcMNPV
infection. One cell line did not support complete virus replication through the late phase of virus growth and did not exhibit
apoptosis. The P. unipuncta cell lines could be distinguished from SF21 and BTI-Tn-5B1-4 cells by their isozyme markers. |
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Keywords: | baculovirus Autographa californica MNPV virus production insect cell culture |
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