Induction of pluripotency in mammalian fibroblasts by cell fusion with mouse embryonic stem cells |
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| Affiliation: | 1. Department of Biomedicine, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan;2. Laboratory of Veterinary Anatomy, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan;3. Amami Laboratory of Injurious Animals, Institute of Medical Science, The University of Tokyo, Kagoshima, Japan;4. Laboratory of Veterinary Developmental Biology, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan;1. Department of Microbiology, Inha University School of Medicine, Incheon, 22212, South Korea;2. Division of Tumor Immunology, National Cancer Center, Goyang, 10408, South Korea;3. Department of Otorhinolaryngology-Head and Neck Surgery, Inha University School of Medicine, Incheon, 22212, South Korea;1. Department of General Practice, The First Hospital of China Medical University, Shenyang, China;2. Department of Nephrology, The First Hospital of China Medical University, Shenyang, China;1. School of Biological Sciences, Department of Molecular & Biomedical Science, Research Centre for Infectious Diseases, University of Adelaide, Adelaide, 5005, Australia;2. Institute for Glycomics, Griffith University Gold Coast Campus, Queensland, 4222, Australia;1. Department of Pharmacy, Hebei Chemical and Pharmaceutical College, Shijiazhuang, 050026, Hebei, PR China;2. Institute of Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, No.18877 Jingshi Road, Jinan, 250062, Shandong, PR China;1. Department of Pharmacology, Peripheral Neuropathy Research Center (PNRC), Dong-A University College of Medicine, Busan, 49201, South Korea;2. Department of Biochemistry, Peripheral Neuropathy Research Center (PNRC), Dong-A University College of Medicine, Busan, 49201, South Korea;3. Department of Neurology, SAIHST, Sungkyunkwan University School of Medicine, Seoul, 06351, South Korea;1. Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul, 05029, South Korea;2. Korea Institute of Dermatological Science, GeneCellPharm Corporation, 375 Munjeong 2(i)-dong, Songpa-gu Seoul, 05836, South Korea |
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| Abstract: | BackgroundCell fusion is a phenomenon that is observed in various tissues in vivo, resulting in acquisition of physiological functions such as liver regeneration. Fused cells such as hybridomas have also been produced artificially in vitro. Furthermore, it has been reported that cellular reprogramming can be induced by cell fusion with stem cells.MethodsFused cells between mammalian fibroblasts and mouse embryonic stem cells were produced by electrofusion methods. The phenotypes of each cell lines were analyzed after purifying the fused cells.ResultsColonies which are morphologically similar to mouse embryonic stem cells were observed in fused cells of rabbit, bovine, and zebra fibroblasts. RT-PCR analysis revealed that specific pluripotent marker genes that were never expressed in each mammalian fibroblast were strongly induced in the fused cells, which indicated that fusion with mouse embryonic stem cells can trigger reprogramming and acquisition of pluripotency in various mammalian somatic cells.ConclusionsOur results can help elucidate the mechanism of pluripotency maintenance and the establishment of highly reprogrammed pluripotent stem cells in various mammalian species. |
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| Keywords: | Pluripotency Cell fusion Embryonic stem cell Induced pluripotent stem cell Mammal |
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