Force Production by a Bundle of Growing Actin Filaments Is Limited by Its Mechanical Properties |
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| Institution: | 1. CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, Grenoble, France;2. CNRS, CHU Grenoble-Alpes, Inserm, TIMC-IMAG, University Grenoble-Alpes, Grenoble, France;3. CNRS, IBDM, Turing Centre for Living Systems, Aix Marseille University, Marseille, France;4. Department of Biochemistry, University of Zurich, Zurich, Switzerland;5. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut;6. Nanobiology Institute, Yale University, West Haven, Connecticut;7. Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut |
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| Abstract: | Bundles of actin filaments are central to a large variety of cellular structures such as filopodia, stress fibers, cytokinetic rings, and focal adhesions. The mechanical properties of these bundles are critical for proper force transmission and force bearing. Previous mathematical modeling efforts have focused on bundles’ rigidity and shape. However, it remains unknown how bundle length and buckling are controlled by external physical factors. In this work, we present a biophysical model for dynamic bundles of actin filaments submitted to an external load. In combination with in vitro motility assays of beads coated with formins, our model allowed us to characterize conditions for bead movement and bundle buckling. From the deformation profiles, we determined key biophysical properties of tethered actin bundles such as their rigidity and filament density. |
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