Identification of two additional plasmodesmata localization domains in the tobacco mosaic virus cell-to-cell-movement protein |
| |
| Institution: | 1. Department of Neurology, Shanxi Provincial People’s Hospital, Taiyuan, Shanxi, 030012, China;2. Department of Neurology, Baoji People’s Hospital, Baoji, Shaanxi,721001, China;3. Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, 100029, China;1. Department of Life Science, Chung-Ang University, Seoul, Republic of Korea;2. Department of Biomedical Engineering, College of Medical Convergence, Catholic Kwandong University, Gangneung, Republic of Korea;3. School of Biological Sciences, Chungbuk National University, Cheongju, Republic of Korea;1. Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA;2. Donald Danforth Plant Science Center, St. Louis, MO 63132, USA;1. University of Florida, Plant Pathology Department, Gainesville, FL 32611, USA;2. Biomedical Sciences Research Complex, University of St Andrews, BMS Building, North Haugh, St Andrews, Fife KY16 9ST, United Kingdom;3. Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom |
| |
| Abstract: | Despite decades of intensive studies, the failure to identify plasmodesmata (PD) localization sequences has constrained our understanding of Tobacco mosaic virus (TMV) movement. Recently, we identified the first PD localization signal (major PLS) in the TMV movement protein (MP), which encompasses the first 50 amino acid residues of the MP. Although the major PLS is sufficient for PD targeting, the efficiency is lower than the full-length TMV MP. To address this efficiency gap, we identified two additional PLS domains encompassing amino acid residues 61 to 80, and 147 to 170 of the MP and showed that these two domains target to PD, but do not transit to adjacent cells. We also demonstrated that the MP61?80 fragment interacts with Arabidopsis synaptotagmin A, which was also shown to interact with the major TMV MP PLS. Therefore, our findings have provided new insights to more fully understand the mechanism underlying plasmodesmal targeting of TMV MP. |
| |
| Keywords: | Plasmodesmata Movement protein Tobacco mosaic virus Plasmodesmata localization signal |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
