Simultaneous Detection of Oseltamivir- and Amantadine-Resistant Influenza by Oligonucleotide Microarray Visualization |
| |
| Authors: | Yingjie Zhang Qiqi Liu Dou Wang Suhong Chen Shengqi Wang |
| |
| Institution: | 1. Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, People''s Republic of China.; 2. Shenzhen Puruikang Biotech Co., Ltd, Shenzhen, People''s Republic of China.; George Mason University, United States of America, |
| |
| Abstract: | Presently, the resistance of Influenza A virus isolates causes great difficulty for the prevention and treatment of influenza A virus infection. It is important to establish a drug-resistance detection method for epidemiological study and personalized medicine in the clinical setting. Consequently, a cost-effective oligonucleotide microarray visualization method, which was based on quantum dot-catalyzed silver deposition, was developed and evaluated for the simultaneous detection of neuraminidase H275Y and E119V; matrix protein 2 V27A and S31N mutations of influenza A (H3N2), seasonal influenza A (H1N1), and 2009 influenza A (H1N1). Then, 307 clinical throat swab specimens were detected and the drug-resistance results showed that 100% (17/17) of influenza A (H3N2) and 100% (259/259) of 2009 influenza A (H1N1) samples were resistant to amantadine and susceptible to oseltamivir; and 100% (5/5) of seasonal influenza A (H1N1) samples were resistant to both amantadine and oseltamivir. |
| |
| Keywords: | |
|
|
