Autofluorescence as a Tool for Structural Analysis of Biofilms Formed by Nonpigmented Rapidly Growing Mycobacteria |
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| Authors: | María-Carmen Mu?oz-Egea María García-Pedrazuela Ignacio Mahillo María Jesús García Jaime Esteban |
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| Affiliation: | aDepartments of Clinical Microbiology;bEpidemiology, IIS-Fundación Jiménez Díaz, Madrid, Spain;cDepartment of Preventive Medicine, Facultad de Medicina, Universidad Autónoma, Madrid, Spain |
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| Abstract: | The structure of biofilms formed by seven nonpigmented rapidly growing mycobacteria, including saprophytes and opportunistic species, was analyzed. Analysis included amount of covered surface, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. Autofluorescence was detected inside and outside cells of all mycobacteria. |
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