Kinetics of IL-7 and IL-15 Levels after Allogeneic Peripheral Blood Stem Cell Transplantation following Nonmyeloablative Conditioning |
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| Authors: | Muriel De Bock Marianne Fillet Muriel Hannon Laurence Seidel Marie-Paule Merville André Gothot Yves Beguin Frédéric Baron |
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| Affiliation: | 1. Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA)–I3., University of Liège, Liège, Belgium.; 2. Department of Statistics, University of Liège, Liège, Belgium.; 3. Department of Medicine, Division of Hematology, University and Centre Hospitalier Universitaire (CHU) of Liège, Liège, Belgium.; CNRS, France, |
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| Abstract: | BackgroundWe analysed kinetics of IL-7 and IL-15 levels in 70 patients given peripheral blood stem cells after nonmyeloablative conditioning.MethodsEDTA-anticoagulated plasma and serum samples were obtained before conditioning and about once per week after transplantation until day 100. Samples were aliquoted and stored at −80°C within 3 hours after collection until measurement of cytokines. IL-7 and IL-15 levels were measured by ELISAs.ResultsMedian IL-7 plasma levels remained below 6 pg/L throughout the first 100 days, although IL-7 plasma levels were significantly higher on days 7 (5.1 pg/mL, P = 0.002), 14 (5.2 pg/mL, P<0.001), and 28 (5.1 pg/mL, P = 0.03) (but not thereafter) than before transplantation (median value of 3.8 pg/mL). Median IL-15 serum levels were significantly higher on days 7 (12.5 pg/mL, P<0.001), 14 (10.5 pg/mL, P<0.001), and 28 (6.2 pg/mL, P<0.001) than before transplantation (median value of 2.4 pg/mL). Importantly, IL-7 and IL-15 levels on days 7 or 14 after transplantation did not predict grade II–IV acute GVHD.ConclusionsThese data suggest that IL-7 and IL-15 levels remain relatively low after nonmyeloablative transplantation, and that IL-7 and IL-15 levels early after nonmyeloablative transplantation do not predict for acute GVHD. |
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