iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks |
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| Authors: | Sylvie Rockel Marcel Geertz Korneel Hens Bart Deplancke Sebastian J. Maerkl |
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| Affiliation: | 1.Laboratory of Biological Network Characterization, Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, 2.Department of Molecular Biology, University of Geneva, Geneva, Switzerland and 3.Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland |
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| Abstract: | Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF–DNA interactions, generating and interrogating an array of 423 full-length Drosophila TFs. With integrated systems-level interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes. |
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