Relationship of Glucocorticoid Receptor Expression in Peripheral Blood Mononuclear Cells and the Cochlea of Guinea Pigs and Effects of Dexamethasone Administration |
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| Authors: | Ling Lu Yanhong Dai Xiaoping Du Wandong She Xiuling Zhang Qin Wu Wenjie Yuan Feng Chen |
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| Institution: | 1. Department of Otolaryngology-Head and Neck Surgery, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.; 2. Hough Ear Institute, Oklahoma City, Oklahoma, United States of America.; 3. Jiangyin People''s Hospital, Southeast University Medical College, Jiangyin, Jiangsu Province, China.; 4. Microbiology Laboratory, Nanjing University Medical School, Nanjing, China.; Universidade Federal do Rio de Janeiro, Brazil, |
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| Abstract: | BackgroundGlucocorticoids (GCs) are widely used to treat sudden sensorineural hearing loss (SSNHL) and significantly improve hearing. However, GC insensitivity has been observed in some patients of SSNHL.ObjectiveTo study the correlation between GR expression in peripheral blood mononuclear cells (PBMCs) and in the cochlea of guinea pigs at mRNA and protein levels.MethodsOne group of guinea pigs received dexamethasone (10 mg/kg/day) intraperitoneally for 7 consecutive days (dexamethasone group), and another group of guinea pigs received normal saline (control group). Real time PCR and Western blotting were used to detect the expression of GR mRNA and GR protein in PBMCs and the cochleae.ResultsThe GR mRNA and GR protein were detected in both PBMCs and the cochlear tissue of guinea pigs. GR mRNA and GR protein levels in PBMCs were positively correlated with those in the cochlea. The expression of GR mRNA and GR protein was significantly increased in the dexamethasone group compared to the control group.ConclusionsLevels of GR mRNA and GR protein in the PBMCs were positively correlated with those in the cochlea of guinea pigs. Systemic dexamethasone treatment can significantly up-regulate GR expression in PBMCs and in the cochlea. Measurement of the GR level in PBMCs could be used as an indicator of GR level in the cochlea. |
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