Unique high sensitivity to heat of axolotl TRPV1 revealed by the heterologous expression system |
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| Institution: | 1. Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Department of Hepatology and Gastroenterology, The Third Central Clinical College of Tianjin Medical University, Tianjin Key Laboratory of Artificial Cells, Artificial Cell Engineering Technology Research Center of Public Health Ministry, Tianjin, China;2. The Third Central Clinical College of Tianjin Medical University, Department of Hepatology and Gastroenterology, Tianjin Key Laboratory of Artificial Cells, Artificial Cell Engineering Technology Research Center of Public Health Ministry, Tianjin, China;3. Department of Hepatopancreatobiliary Surgery, The Affiliated Tumor Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China;1. Regenerative and Restorative Medicine Research Center, REMER, Istanbul Medipol University, Istanbul, Turkey;2. Department of Computer Engineering, Faculty of Engineering, Mugla Sitki Kocman University, Mugla, Turkey;3. Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC, 20007, USA;4. Acibadem Labmed Research and Development Center, Istanbul, Turkey;5. Department of Biomedical Engineering, Faculty of Engineering, Istanbul Medipol University, Istanbul, Turkey;6. Department of Physiology, International School of Medicine, Istanbul Medipol University, Istanbul, Turkey;7. Department of Biochemistry, School of Medicine, Istanbul Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey;8. Department of Medical Biology, School of Medicine, Mugla Sitki Kocman University, Mugla, Turkey;1. Molecular & Developmental Complexity Group, Unit of Advanced Genomics, UGA-CINVESTAV, Irapuato, Mexico;2. Department of Biochemistry, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, Mexico;3. Departamento de Genética del Desarrollo y FisioloMéxico Citygía Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, AP 510-3, Cuernavaca, Mor, 62250, Mexico;4. Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, DF, 04510, Mexico;1. Amphibian Research Center, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8526, Japan;1. School of Biological Sciences, National Institute of Science Education and Research, Khordha, Jatni, Odisha, 752050, India;2. Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400094, India;3. Frozen Semen Bank, Animal Husbandry and Veterinary Services -Odisha, Khapuria, Cuttack, 753010, Odisha, India;4. Central Avian Research Institute, Bhubaneswar, 751003, India;5. Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, India;6. Department of Genetics & Molecular Biology in Botany, Institute of Botany, Christian-Albrechts-University at Kiel, 24118, Germany;7. Rajiv Gandhi Centre for Biotechnology, Thycaud, Poojappura, Trivandrum, Kerala 695014, India;1. Division of Embryology, Amphibian Research Center, Hiroshima University, Higashihiroshima 739 8526, Japan;2. Section on Molecular Morphogenesis, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, MD, USA |
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| Abstract: | The thermosensation mechanism plays critical roles in various animals living in different thermal environment. We focused on an axolotl, which is a tailed amphibian originally from Lake Xochimilco area in the Vally of Mexico, and examined its behavior response to heat stimulation. Mild heat at 33 °C induced noxious locomotive activity to axolotls, but the noxious response of another tailed amphibian, Iberian ribbed newt, was not observed at 33 °C. To explore the mechanism for the temperature sensitivity of axolotls, we isolated a cDNA of TRPV1. Using the degenerate primer PCR method, we identified the DNA fragment encoding axolotl TRPV1 (axTRPV1), and then cloned a full-length cDNA. We studied the chemical and thermal sensitivities of axTRPV1 by two-electrode voltage clamp method using Xenopus oocyte expression system. Capsaicin, acid, and 2-aminoethoxydiphenylborane apparently activated axTRPV1 channels in a dose-dependent manner. The analysis of thermal sensitivity showed that axTRPV1 was significantly activated by heat but not by cold. The average temperature threshold for heat-activation was 30.95 ± 0.12 °C. This thermal activation threshold of axTRPV1 is unique and significantly low, when compared with the known thresholds of TRPV1s from various animals. Further, this threshold of axTRPV1 is well consistent with the observation of heat-induced behavior of axolotls at 33 °C, demonstrating that axolotl shows noxious response to mild heat mediated through axTRPV1. |
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| Keywords: | Transient receptor potential Channel Neuron Thermosensing Amphibian TRP"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "_":"transient receptor potential axTRPV1"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"axolotl TRPV1 2-APB"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"2-aminoethoxydiphenyl borate |
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