Estimation of proteins in the presence of polyethylenimine |
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Authors: | Vandana Gupta Sunil Nath Subhash Chand |
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Institution: | (1) Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi, 110 016, India |
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Abstract: | Polyethylenimine (PEI) interferes strongly in all commonly employed protein estimation assays. Here, a strategy to remove this interference is developed. Polyethylenimine is selectively removed by adsorption from a mixture containing PEI and lysozyme using carboxymethyl-cellulose, a cation-exchange resin between an ionic strength of 0.35–1 M (pH 6.0), conditions under which lysozyme remains in solution and can be accurately estimated. |
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Keywords: | adsorption bioseparation carboxymethyl-cellulose interference ion-exchange resin polyethylenimine proteins |
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