Thermal resistance of partially purified proteinase of Pseudomonas fluorescens P-26 |
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Authors: | VK Uplacksh DK Mathur RK Malik |
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Institution: | Dairy Microbiology Division, National Dairy Research Institute, Karnal, India |
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Abstract: | Heat resistance of the Pseudomonas fluorescens P-26 proteinase in terms of D -value was studied in whole milk, skim milk, whey and 0.05 mol 1-1 phosphate buffer at 72.5, 130, 135, 140, 145 and 150°C subsequent to its partial purification through (NH4)2 SO4 precipitation (45–65% saturation) and solvent fractionation with 1.0 to 2.0 volumes of isopropanol. The D -value was maximum for the proteinase at all temperatures when determined in whole milk ( D 150= 0.088). |
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