Germ cell-sertoli cell interactions and production of testosterone by purified Leydig cells from mature rat |
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Authors: | N Boujrad J M Guillaumin P Bardos M T Hochereau de Reviers M A Drosdowsky and S Carreau |
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Institution: | 1 URA C.N.R.S. 609 Biochimie, CHU Côte de Nacre, 14033, Caen Cédex, France 2 Immunologie, CHU Bretonneau, Tours, France 3 INRA, Nouzilly, France |
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Abstract: | The addition of seminiferous tubule (ST) culture medium (STM) prepared from testes of either busulfan-treated (Bus) or cryptorchid (Cryp) or genetically sterile (hd) rats, to Percoll purified Leydig cells leads to a further increase of LH-stimulated testosterone (T) output (26, 43 and 14%, respectively). Taking into account that the Sertoli cell number per cm of ST is 2.6, 1.8 and 1.4-fold greated in Bus, Cryp and hd rats than in controls, the above STM effects on T output, expressed per 106 Sertoli cells are in fact lower (63, 44 and 43%, respectively) that those of control STM. Similar results have been obtained for the STM transferrin levels which are decreased, 74, 67 and 45%, respectively in Bus, Cryp and hd animals. So, it is likely that the Sertoli cell secretion of both the paracrine factor involved on Leydig cell T production and the transferrin is influenced mainly by spermatids and to a lesser extent by spermatocytes of mature rat testis. |
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