| 固氮酶结构基因在联合固氮菌Klebsiellaplanticola19—1细胞中的定位 |
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| 引用本文： | 李久蒂 靳红帆. 固氮酶结构基因在联合固氮菌Klebsiellaplanticola19—1细胞中的定位[J]. Acta Botanica Sinica, 1994, 36(10): 755-758 |
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| 作者姓名： | 李久蒂 靳红帆 |
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| 作者单位： | 中国科学院植物研究所!北京100044 |
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| 摘 要： | 用Ｋｌｅｂｓｉｅｌｌａ ｐｎｅｕｍ ｏｎｉａｅｎｉｆＨＤＫ 为探针与Ｋｌｅｂｓｉｅｌｌａ ｐｌａｎｔｉｃｏｌａ １９－１ ＤＮＡ Ｓｏｕｔｈｅｒｎ 杂交表明，Ｋ．ｐｌａｎｔｉｃｏｌａ １９－１ 中存在与ｎｉｆＨＤＫ 同源的片段。用凝胶内溶菌及电场倒转技术检测在Ｋ．ｐｌａｎｔｉｃｏｌａ １９－１ 中存在一大质粒。用Ｋ．ｐｎｅｕｍ ｏｎｉａｅ ｎｉｆ ＨＤＫ 探针与大质粒ＤＮＡＳｏｕｔｈｅｒｎ 杂交证明固氮酶结构基因定位于大质粒
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| 关 键 词： | Klebsiellaplanticola 固氮酶结构基因 大质粒 基因定位 |
LOCALIZATION OF NITROGEN FIXATION GENE IN AN ASSOCIATED NITROGEN FIXING BACTERIA KLEBSIELLA PLANTICOLA 19 1 |
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| Li Jiu di and Jin Hong fan. LOCALIZATION OF NITROGEN FIXATION GENE IN AN ASSOCIATED NITROGEN FIXING BACTERIA KLEBSIELLA PLANTICOLA 19 1[J]. , 1994, 36(10): 755-758 |
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| Authors: | Li Jiu di and Jin Hong fan |
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| Abstract: | A large plasmid in Klebsiella planticola 19 1 was identified from lysis of bacteria in an agarose gel and using a technique of electrical field overturn.The presence of nif HDK like gene and localization of nif HDK like gene in the large plasmid were confirmed by Southern hybridization with 32 P labelled nif HDK probe. |
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| Keywords: | Klebsiella planticola nif HDK Large plasmid Localization of gene |
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