花粉高尔基囊泡与牛脑微管的体外结合

把从榛木（Ｃｏｒｙｌｕｓａｖｅｌｌａｎａ Ｌ．）花粉中分离得到的高尔基囊泡与经高度纯化并聚合好的牛脑微管进行体外组合，然后于１．５ ｍ ｏｌ／Ｌ的蔗糖层上进行超离心，对其沉淀物进行ＳＤＳ－聚丙烯酰胺凝胶电泳和电镜负染。结果表明，花粉高尔基囊泡可以结合到牛脑微管上，证明植物花粉的高尔基囊泡与动物细胞的某些细胞器一样，也与细胞骨架的主要组成之一——微管具有结构上的紧密联系。花粉高尔基囊泡与牛脑微管的体外结合能力，受１０ ｍ ｍ ｏｌ／ＬＡＴＰ和０．５ ｍ ｏｌ／ＬＫＣｌ的影响，但不受５ ｍ ｍ ｏｌ／Ｌ ＡＭＰ－ＰＮＰ的影响，说明两者结合可能是通过高尔基囊泡表面与ＡＴＰ有关的某种外周膜蛋白来完成的。

BINDING OF POLLEN GOLGI VESICLES TO CALF BRAIN MICROTUBULE IN VITRO

The Golgi vesicles isolated from the pollen of Corylus avellana were incubated with the microtubules polymerized from highly purified calf brain tubulin. After ultracentrifugation on a layer of 1 5 mol/L sucrose, the pellets were assayed with SDS FAGE and negative staining.The results showed that, in vitro, the pollen Golgi vesicles were able to bind onto calf brain microtubules.The microtubule binding ability was suppressed by 10 mmol/L ATP and 0 5 mol/L KCl, but not by 5 mmol/L AMP PNP, suggesting that the binding may result from some ATP related peripheral membrane proteins.