Vasoactive intestinal peptide: levels and functional receptors in rat brain before and after weaning. |
| |
Authors: | P Robberecht M Deschodt-Lanckman P De Neef J Christophe |
| |
Affiliation: | Department of Biochemistry and Nutrition, School of Medicine, Université Libre de Bruxelles, Boulevard de Waterloo 115, B-1000 Brussels, Belgium |
| |
Abstract: | The average level of VIP was found to be 17 pmol/g wet weight in the brain of the newborn rat. This level ramained constant during the first two weeks after birth then increased progressively to 40 pmol/g wet weight at 20 days, a value comparable to that found in adult animals. VIP was already able to stimulate brain membrane adenylate cyclase activity at birth. The stimulation with 10?6 M VIP allowed a 2.5-fold increase in basal activity in membranes from 1 to 14-days-old pups as compared to a 1.7-fold stimulation in membranes from adult brain. The apparent activation constant for VIP adenylate cyclase stimulation was 4.10?7 M at all ages. The efficiency of VIP activation amounted to as much as 70% of that of fluoride at birth and to 35% only of fluoride activation in brain membranes from adult rats. |
| |
Keywords: | VIP vasoactive intestinal peptide |
本文献已被 ScienceDirect 等数据库收录! |