Detoxification mechanisms for 1,2,4-benzenetriol employed by a Rhodococcus sp. BPG-8 |
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Authors: | S Armstrong T R Patel M Whalen |
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Institution: | (1) Department of Biology and Biochemistry, Memorial University of Newfoundland, A1B 3X9 St. John's, NF, Canada |
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Abstract: | A Rhodococcus sp. BPG-8 produces 1,2,4-benzenetriol during the transformation of resorcinol by phloroglucinol induced cell-free extract. The oxidation of 1,2,4-benzenetriol to 2-hydroxy-1,4-benzoquinone produces superoxide radicals that may have potential deleterious effects on cellular integrity. It has been shown that both superoxide dismutase (SOD) and catalase retard the autoxidation of 1,2,4-benzenetriol to 2-hydroxy-1,4-benzoquinone. Termination of the free radical chain reaction between superoxide radical and 1,2,4-benzenetriol seems to prevent this autoxidation. A NAD(P)H-dependent reductase appears to convert the 2-hydroxy-1,4-benzoquinone back to 1,2,4-benzenetriol. Both of these mechanisms appear to stabilize 1,2,4-benzenetriol so that it may be cleaved by meta cleavage enzymes. The enzymes responsible for the stabilization of 1,2,4-benzenetriol appear not to be inducible. |
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Keywords: | 1 2 4-Benzenetriol 2-hydroxy-1 4-benzoquinone Rhodococcus BPG-8 NAD(P)H-Dependent reductase |
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