The nusG gene of Streptomyces griseus: Cloning of the gene and analysis of the A-factor binding properties of the gene product |
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Authors: | Sabine Kuberski,Thomas Kasberg,Jü rgen Distler |
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Affiliation: | Institut für Chemische Mikrobiologie, FB9, BUGH Wuppertal, Gauss-Str. 20, D-42097 Wuppertal, FRG |
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Abstract: | Abstract The nusG gene of Streptomyces griseus was cloned and the nucleotide sequence determined. It encodes a protein with an identify of 76% to the reported receptor (VbrA) for VB-C, an autoregulatory factor in Streptomyces virginae . NusG protein was expressed in Escherichia coli . However, no binding activity for A-factor, an butyrolactone autoregulator in S. griseus very similar to VB-C, could be detected. The nusG gene of S. griseus does not seem to encode the A-factor-binding protein. |
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Keywords: | A-factor A-factor binding protein nusG gene Streptomyces griseus |
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