Development of an improved <Emphasis Type="Italic">Pseudoalteromonas haloplanktis</Emphasis> TAC125 strain for recombinant protein secretion at low temperature |
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| Authors: | Ermenegilda Parrilli Daniela De Vizio Claudia Cirulli Maria Luisa Tutino |
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| Institution: | 1.Dipartimento di Chimica Organica e Biochimica,Università degli studi di Napoli Federico II – Complesso Universitario M.S,Napoli,Italia;2.Facoltà di Scienze Biotecnologiche, Università degli studi di Napoli Federico II,Napoli,Italia;3.Dipartimento di Biotecnologie e Bioscienze dell,Università Milano-Bicocca,Milano,Italia |
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| Abstract: | Background In a previous paper, we reported the accomplishment of a cold gene-expression system for the recombinant secretion of heterologous
proteins in Pseudoalteromonas haloplanktis TAC125. This system makes use of the psychrophilic α-amylase from P. haloplanktis TAB23 as secretion carrier, and allows an effective extra-cellular addressing of recombinant proteins. However, Pseudoalteromonales are reported to secrete a wide range of extra-cellular proteases. This feature works against the efficiency of the cold-adapted
secretion system, because of the proteolytic degradation of recombinant products. The aim of this study is the construction
of a P. haloplanktis TAC125 mutant strain with reduced extra-cellular proteolytic activity. |
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