Xanthomonas campestris pv. campestris lpsI and lpsJ genes encoding putative proteins with sequence similarity to the α- and β-subunits of 3-oxoacid CoA-transferases are involved in LPS biosynthesis |
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Authors: | Dorothee Steinmann R Köplin A Pühler Karsten Niehaus |
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Institution: | Lehrstuhl für Genetik, Universit?t Bielefeld, Postfach 10 01 31, D-33501 Bielefeld, Germany Tel. +49-(0)521-106-5632; Fax +49-(0)521-106-5626 e-mail: japps@genetik.uni-bielefeld.de, DE
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Abstract: | A 2.1-kb SmaI-EcoRI DNA fragment upstream of the xanA and xanB genes of Xanthomonas campestris pv. campestris carries two ORFs encoding putative proteins with sequence similarities to the α- and β-subunits of 3-oxoacid-CoA transferases.
The two ORFs were termed lpsI and lpsJ because strains carrying appropriate mutations showed an autoagglutination phenotype and because lipopolysaccharides of these
mutant strains were altered according to silver-stained polyacrylamide gels. The monosaccharide composition of the exopolysaccharide
xanthan produced by lpsI and lpsJ mutants remained unchanged.
Received: 29 March 1997 / Accepted: 21 July 1997 |
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Keywords: | Xanthomonas campestris pv campestris lpsI lpsJ 3-Oxoacid CoA-transferase Xanthan Lipopolysaccharide |
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