Thidiazuron-induced high-frequency shoot organogenesis from leaf-derived callus of ia medicinal climber, <Emphasis Type="Italic">Tylophora Indica</Emphasis> (Burm. F.) merrill |
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Authors: | Email author" target="_blank">T?Dennis?ThomasEmail author Boban?Philip |
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Institution: | (1) Postgraduate and Research Department of Botany, St. Thomas College, Pala, Kottayam (Dt.), Arunapuram (P.O), PIN 686 574 Kerala, India |
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Abstract: | Summary
Tylophora indica (Burm. f.) Merrill is a threatened medicinal climber distributed in the forests of northern and peninsular India. An efficient
and reproducible protocol for high-frequency callus regeneration from immature leaf explants of T. indica was developed. Organogenic callus formation from immature leaf pieces was obtained by using Murashige and Skoog (MS) medium
supplemented with 7 μM 2,4-dichlorophenoxyacetic acid and 1.5 μM 6-benzyladenine. On this medium 92% explants produced callus. The optimal hormone combination for plantlet regeneration was
8 μM thidiazuron, at which shoot regeneration was obtained from 100% of the cultures, with an average of 66.7 shoots per culture.
Histological studies of the regenerative callus revealed that shoot buds were originated from the outermost regions. For root
formation, half-strength MS medium supplemented with 3 μM indole-3-butyric acid was used. Plants were transferred to soil, where 92% survived after 3 mo. of acclimatization. |
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Keywords: | acclimatization Asclepiadaceae growth regulators in vitro regeneration micropropagation rooting |
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