A sensitive assay for the reverse reaction of the first histidine biosynthetic enzyme. |
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| Authors: | J Kleeman S M Parsons |
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| Affiliation: | Department of Chemistry, University of California, Santa Barbara, California 93106 USA |
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| Abstract: | A method capable of specifically detecting low levels of adenosine triphosphate phosphoribosyltransferase (EC 2.4.2.17) is given. It is based on conversion of the usual product of the enzyme, N1-(5′-phospho-d-ribosyl)adenosine triphosphate to ATP, which is detected by luciferase luminescence utilizing a liquid scintillation spectrophotometer. The assay is linear in enzyme concentration down to a lower limit of at least 55 pg enzyme/0.2 ml. |
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