| 一株水禽流感病毒分离株表面膜蛋白基因的序列分析 |
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| 引用本文： | 张评浒,薛峰,唐应华,钱忠明,郝贵杰,刘慧谋,刘秀梵.一株水禽流感病毒分离株表面膜蛋白基因的序列分析[J].Virologica Sinica,2005,20(5):555-557. |
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| 作者姓名： | 张评浒 薛峰 唐应华 钱忠明 郝贵杰 刘慧谋 刘秀梵 |
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| 作者单位： | 扬州大学农业部畜禽传染病学重点开放实验室 江苏扬州225009
(张评浒，薛峰，唐应华，钱忠明，郝贵杰，刘慧谋)，扬州大学农业部畜禽传染病学重点开放实验室 江苏扬州225009(刘秀梵) |
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| 基金项目： | 国家科技攻关计划项目(2004BA519A02)，江苏省属高校重大基础研究项目(05KJA23016) |
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| 摘 要： | 禽流感(AvianInfluenza,AI)是由A型流感病毒所引起的各种家禽及野生禽类感染和/或疾病综合征1]。根据其表面糖蛋白血凝素蛋白(Hemag glutinin,HA)和神经氨基酸酶(Neuraminidase,NA)的抗原关系不同,目前可分为16种HA亚型和9种NA亚型2,3]。近几年来,南亚国家屡有禽流感病毒突破种间屏障作用,直接感染人类或其它哺乳动物,甚至致人死亡事件4～6]的情况发生,因而赋予了禽流感全新的公共卫生学意义。因此,准确的了解和把握水禽,尤其是家养水禽的流感生态,对预防禽流感的发生具有非常重要的现实意义。为了防患于未然,近年来扬州大学农业…
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| 关 键 词： | 禽流感病毒 H11N2亚型 HA NA 剪切位点 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-05-23 |
Molecular Analysis of the Surface Glycoprotein Genes of an Aquatic Bird Influenza Virus |
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| ZHANG Ping-hu, XUE Feng, TANG Ying-hua, QIAN Zhong-ming, HAO Gui-jie, LIU Hui-mou,LIU Xiu-fan.Molecular Analysis of the Surface Glycoprotein Genes of an Aquatic Bird Influenza Virus[J].中国病毒学(英文版),2005,20(5):555-557. |
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| Authors: | ZHANG Ping-hu XUE Feng TANG Ying-hua QIAN Zhong-ming HAO Gui-jie LIU Hui-mou LIU Xiu-fan |
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| Institution: | Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou, 225009,China |
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| Abstract: | Several H11N2 subtype of Avian influenza A viruses were isolated from aquatic birds in live bird markets when we surveyed the ecology of the influenza in East China for more than two years and identified by specific RT-PCR.The hemagglutinin(HA) and neuraminidase(NA) genes of one representative virus named A/Duck/Yangzhou/44/2002(H11N2)(DYZ/44/02) was sequenced.The results showed the HA nucleotide sequence of DYZ/44/02 has high identity with Dk/England/56(H11N6) and the NA nucleotide sequence of DYZ/44/02 has more than 95% sequence homology with Dk/Hokkaido/49/98(H9N2).Sequencing and phylogenetic analysis of the N2 NA genes of DYZ/44/02 revealed that the NA gene of DYZ/44/02 has close relationships with that of Ck/Korea/MS96/96-like H9N2 virus and are distinct from those of Ck/Beijing/94(H9N2).The sequence of cleavage site of DYZ/44/02 consists of a single arginine,as is the case with most other hemagglutinins exhibiting low susceptibility to proteolytic activation. |
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| Keywords: | Avian influenza virus H11N2 subtype HA NA Cleavage site |
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