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Overproduction and purification of initiation factor IF2 and pNUSA proteins from a recombinant plasmid bearing strain |
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| Authors: | J Dondon J A Plumbridge J W Hershey M Grunberg-Manago |
| Affiliation: | 1. Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France |
| Abstract: | The genes for translational initiation factor, IF2 and pNusA have been cloned into a plasmid vector where they are placed under the control of the inducible lambdapL promoter and the c1857 thermosensitive repressor. When a strain carrying this plasmid is heat induced, IF2 alpha, IF2 beta and pNusA are overproduced 15 to 20 fold. This has allowed us to purify the IF2 and NusA proteins in large amounts. |
| Keywords: | surproduction purification facteur d'initiation pNUSA plasmide recombinant overproduction purification initiation factor pNUSA recombinant plasmid EDTA (ethylenediaminetetraacetic acid) Me (mercaptoethanol) PMSF (phenylmethylsulfonylfluoride) SDS (sodium dodecylsulfate) Tris (trishydroxymethylaminomethane) DEAE (diethylaminoethyl) PC (phosphocellulose) |
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