Rubisco and some chaperone protein responses to water stress and rewatering at early seedling growth of drought sensitive and tolerant wheat varieties |
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Authors: | Klimentina Demirevska Lyudmila Simova-Stoilova Valya Vassileva and Urs Feller |
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Institution: | (1) Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bdg. 21, Sofia, 1113, Bulgaria;(2) Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland |
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Abstract: | Four wheat varieties differing in their drought tolerance were subjected to severe but recoverable water stress at seedling
stage. Growth parameters, leaf water deficit (WD) and electrolyte leakage (EL) were used to evaluate the stress intensity
and the extent of recovery. The physiological response of the varieties was quite similar under severe drought. Leaf protein
patterns and levels of some individual proteins relevant to ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) maintenance
were studied in control, stressed and recovering plants by electrophoresis and immunoblotting. The bands representing Rubisco
large subunit (RLS), N- and C-terminus of RLS, Rubisco activase (RA) and Rubisco binding protein (RBP, cpn 60), as well as
the chaperone and proteolytic subunits of the Clp protease complex were identified using polyclonal antibodies. Under drought
conditions RLS, Clp proteases and especially RBP were enhanced, whereas the RA band was only slightly affected. The drought
tolerant varieties had higher RBP content in the controls and drought treated plants. Its concentration could be a potential
marker for drought tolerance. |
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Keywords: | Chaperones Early seedling growth Drought stress and recovery Triticum aestivum L Rubisco |
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