Bactericidal Activity of Catecholamine Copper Complexes |
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Authors: | J Aronovitch D Godinger G Czapski |
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Institution: |
a Department of Molecular Biology, School of Medicine, Jerusalem, Israel
b Department of Physical Chemistry, The Hebrew University, Jerusalem, Israel |
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Abstract: | Washed or growing E. coli cells are killed by epinephrine, norepinephrine or dopamine in the presence of non lethal concentrations of Cu(II). Killing is enhanced by anoxia and by sublethal Concentrations of H2O1. The rate of killing is proportional to the rate of catecholamine oxidation. The copper epinephrine complex binds to E. coli cells, induces membrane damage and depletion of the cellular ATP pool. The cells may be partially protected by SOD or catalase but not by OH radical scavengers. Addition of H2O2 to cells which were sensitized by preincubation with the epinephrine-copper complex, causes rapid killing and DNA degradation. Sensitized cells are not protected by BSA. |
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Keywords: | Catecholamines copper bactericidal effect ATP membrane damage DNA degradation |
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