The influence of culture medium composition on the incidence of chromosomal breakage |
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| Authors: | Marianne Keck Ingrid Emerit |
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| Affiliation: | (1) Laboratory of Cytogenetics, Institut biomédical des Cordeliers Université Pierre et Marie Curie Paris, 15, rue de l'Ecole de Médecine, F-75014 Paris, France;(2) Present address: the Institut für Biologie, Forschungszentrum Seibersdorf, Austria |
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| Abstract: | Summary In patients with chromosomal instability and in healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC Medium 199, Eagle's Minimum Essential Medium, or RPMI 1629. The importance of the choice of culture medium for mutagenicity testing and studied of so-called spontaneous breakage is shown. Cultures incubated with TC Medium 199 showed the highest chromosomal breakage incidence. |
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