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A Method for the Rapid Growth in Culture of Gametophytes of Equisetum arvense with Antheridia
Authors:Kuriyama  Akira; Hojoh  Toshiyuki; Sugawara  Yasutake; Matsushima  Hisashi; Takeuchi  Masayuki
Institution:Laboratory of Plant Morphogenesis, Department of Regulation Biology, Faculty of Science, Saitama University Urawa, 338 Japan
Abstract:Rapid growth in culture of Equisetum arvense gametophytes wasobtained using Murashige-Skoog's medium plus 3% (w/v) sucroseand continuous illumination. In darkness, growth was reducedand chlorophyll synthesis markedly inhibited. Antheridia formedon the top margin of gametophytes in light and darkness butarchegonia were not observed in either case. 1Present address: Interdisciplinary Research Institute of EnvironmentalSciences, Higashi-yanagi-cho, Nishi-iru, Shichihon-matsu, Itsutsuji-dori,Kamigyo-ku, Kyoto, 602 Japan. (Received June 5, 1989; Accepted September 12, 1989)
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